Version 1.x of NEBuilder is NOT BEING UPDATED ANY FURTHER, but will remain available if you need it. Please use the latest supported version instead at https://nebuilder.neb.com.

NEBuilder Assembly Tool can be used to design primers for your NEBuilder HiFi DNA or Gibson Assembly reactions, based on the entered fragment sequences and the polymerase being used for amplification.

Basic Usage:

1. Set preferences, including the number of fragments and the PCR enzyme. Change settings at any time and the results will be instantly updated.
2. Build your construct by adding fragments to your assembly. Fragments can also be reordered or deleted, with immediate updating of the results.

NEBuilder Assembly Tool provides a list of required primers, recommended annealing temperatures, the final sequence of your assembled construct and a summary of the design.

Additional Information:

• Find more about NEBuilder HiFi DNA Assembly or Gibson Assembly.
• NEBuilder HiFi DNA Assembly Cloning Kit / Master Mix Manual (PDF)
• Gibson Assembly Cloning Kit Manual (PDF)
• Gibson Assembly Master Mix Manual (PDF)

Fragment arrangement

Notes

Required Primers